| Subject: |
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X chromosome inactivation analysis |
| Name: |
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Radek Sima |
| Date Posted: |
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Sep 10, 05 - 3:09 AM |
| Email: |
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sima@medima.cz |
| Message: |
|
Hi, we try to analyse clonality of some formalin fixed paraffin embeded tissue through X chromosome inactivation pattern assay on HUMARA locus (trinucleotide STR). In many cases we observe band shift among digested and nondigested DNA (even using Hha I or HpaII enzyme) on polyAA . We have already tried several different isolation methods and many different PCR conditions including addition of some PCR additives or enhanceres but without any reliable solving of our problem. Can anybody advice us, where can be the problem or what to try? Thank you. |
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